Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Mar;31(3):346-9.

[In vitro generation of insulin-producing cells from the neonatal rat bone marrow mesenchymal stem cells].

[Article in Chinese]

Li X¹, Huang H¹, Liu X¹, Xia H², Li M¹.

Abstract

Objective To observe the differentiation of the neonatal rat bone marrow mesenchymal stem cells (MSCs) into insulin-producing cells and detect the expressions of insulin, pancreatic duodenal homebox-1 (PDX-1) and nestin. Methods MSCs were isolated from the neonatal rats and cultured in the modified medium composed of 10 μg/L human epidermal growth factor (EGF), 10 μg/L basic fibroblast growth factor (bFGF), 10 μg/L hepatocyte growth factor (HGF), 10 μg/L human B cell regulin, 20 mmol/L nicotinamide and 20 g/L B27. After the induction, the mRNA expressions of insulin, PDX-1 and nestin were examined by reverse transcription-PCR, and the insulin, PDX-1 and nestin protein levels were detected by immunocytochemistry. Results The insulin and PDX-1 mRNA expressions increased and the nestin mRNA expression decreased in the differentiation of the neonatal rat MSCs into insulin-producing cells. The nestin, PDX-1 and insulin proteins were co-expressed in insulin-producing cells. Conclusion MSCs can be induced to differentiate into insulin-producing cells.