Am J Transl Res. 2016 Jun 15;8(6):2562-74. eCollection 2016.

Growing a whole porcine liver organ ex situ for six hours without red blood cells or hemoglobin.

Dong J¹, Xia L², Shen H¹, Bian C³, Bao S⁴, Zhang M⁵, Du Y⁶, Dai Y¹, Zhao L¹, Xu Y⁵, Xiong Q³, Xu J⁷, Xu L⁸.

Abstract

Liver transplantation is an effective approach to end-stage liver disease. Shortage of donor liver and increased waiting time for liver transplantation necessitate the development of an organ culture system by which livers can be cultured and maintained ex situ for a prolonged period of time. The aim of this work is to test whether cell culture condition in vitro could be used to culture whole livers ex situ without the use of erythrocytes. Twelve castrated male land race/farm young porcine livers were exposed to 30 min warm ischemia and 30 min cold perfusion. Livers were isolated and connected to an Ex situ liver culture system using a standard culture medium RPMI1640 supplied with 10% of fetal bovine serum and sufficient dissolved oxygen under a normothermic condition for 6 hours. Metabolic biomarkers, bile and urea production, hepatic cell viability and histology analysis of biopsies were examined and newly proliferated hepatic cells labeled by BrdU were analyzed after 6 hours ex situ culture. The results from biochemical assays and histology analysis indicate that livers after the organ culture still maintain the full function.

CONCLUSIONS:

Our data demonstrate that the liver culture system established in this work can be used to culture whole livers ex situ in the absence of erythrocytes.