Cell Transplant. 2016 Jun 9. [Epub ahead of print]

Human Urinary Epithelial Cells as a Source of Engraftable Hepatocyte-like Cells using Stem Cell Technology.

Sauer V, Tchaikovskaya T, Wang X, Li Y, Zhang W, Tar K, Polgar Z, Ding J, Guha C, Fox IJ, Roy-Chowdhury N, Roy-Chowdhury J.

Abstract

Although several types of somatic cells have been reprogrammed to induced pluripotent stem cells (iPSC) and then differentiated to hepatocyte-like cells (iHep), the method for generating such cells from renal tubular epithelial cells shed in human urine and transplanting them in animal livers has not been described systematically. We report reprogramming of human urinary epithelial cells to iPSCs and subsequent hepatic differentiation, followed by a detailed characterization of the newly generated iHeps. The epithelial cells were reprogrammed into iPSCs by delivering the pluripotency factors OCT3/4, SOX2, KLF4 and MYC using methods that do not involve transgene integration, such as nucleofection of episomal (oriP/EBNA-1) plasmids or infection with recombinant Sendai viruses. After characterization of stable iPS cell lines, a 3-step differentiation toward hepatocytes was performed. The iHeps expressed a large number of hepatocyte-preferred genes, including nuclear receptors that regulate genes involved in cholesterol homeostasis, bile acid transport and detoxification. MicroRNA profile of the iHeps largely paralleled that of primary human hepatocytes. The iHeps engrafted into the livers of Scid mice transgenic for mutant human SERPINA1 after intrasplenic injection. Thus, urine is a readily available source for generating human iHeps that could be potentially useful for disease modeling, pharmacological development and regenerative medicine.