Exp Hematol. 2016 Aug 11. pii: S0301-472X(16)30545-8. doi: 10.1016/j.exphem.2016.08.001. [Epub ahead of print]

Immunological properties of bone marrow microenvironment one year after allogeneic hematopoietic stem cell transplantation.

Ciomber A¹, Mitrus I², Fidyk W³, Smagur A³, Chwieduk A³, Glowala-Kosinska M³, Czerw T³, Sobczyk-Kruszelnicka M³, Mendrek W³, Sadus-Wojciechowska M³, Najda J³, Holowiecki J³, Giebel S³.

Abstract

Regeneration of bone marrow microenvironment after transplantation of allogeneic hematopoietic stem cells is poorly explored. The goal of this study was to investigate this process focusing on immunological factors - concentration of selected cytokines, expression of immunosuppressive proteins CD47 and CD274 on hematopoietic stem cells and frequency of T regulatory lymphocytes. Bone marrow samples were collected before, on the day of transplantation and during one year follow-up. As a control group we used bone marrow from healthy donors. Prior to the conditioning, the percentage of Tregs and concentration of IL-10 in bone marrow of patients were higher than in healthy donors. Conditioning regimen resulted in increased concentration of IFN-gamma and expression of CD274 on HSCs. 28 days after transplantation level of Tregs, expression of CD47 and concentration of IL-10 and LAP-1 were increased compared to the period before conditioning. Starting from day 100 after the transplantation the microenvironment tended to normalize - Tregs level and the concentration of most of the cytokines were similar to values in bone marrow of healthy donors.