Arch Gynecol Obstet. 2016 Dec 7. [Epub ahead of print]

Biological and biomechanical analysis of two types of mesenchymal stem cells for intervention in chemotherapy-induced ovarian dysfunction.

Pan Y¹, Zhang L¹, Zhang X¹, Hu C¹, Liu R².

Abstract

PURPOSE:

The study aim was to investigate the biological and biomechanical features of a chemotherapy-induced ovarian dysfunction (CIOD) rat model after intervention with human umbilical cord mesenchymal stem cells (UC-MSCs) and human amniotic mesenchymal stem cells (h-AMSCs), thus providing a biological and biomechanical research basis for clinical treatment.

METHODS:

The serum levels of estradiol (E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and vascular endothelial growth factor (VEGF) in the rat CIOD models were evaluated on the 14th, 30th, 60th, and 90th day of intervention with h-AMSCs and UC-MSCs. In addition, the ovaries in each group were sampled on the 14th and 90th day of intervention for tissue morphology and tensile testing.

RESULTS:

The serum levels of E2, LH, and VEGF in the h-AMSC and UC-MSC groups were greater than in the model group, but the serum FSH level was less than in the model group, and the differences were significant (P < 0.05); the maximum tensile stress and maximum strain in h-AMSC and UC-MSC groups were significantly greater than in the model group (P < 0.05).

CONCLUSIONS:

UC-MSC and h-AMSC intervention restored damaged ovarian morphology, elasticity, and toughness to a certain extent, and ovarian function showed some recovery.