Biotechnol Lett. 2017 Mar 3. doi: 10.1007/s10529-017-2315-4. [Epub ahead of print]

Human umbilical cord mesenchymal stem cells alleviate inflammatory bowel disease through the regulation of 15-LOX-1 in macrophages.

Mao F¹, Wu Y¹, Tang X², Wang J¹, Pan Z¹, Zhang P¹, Zhang B¹, Yan Y¹, Zhang X¹, Qian H¹, Xu W³.

Abstract

OBJECTIVE:

To investigate the role of human umbilical cord mesenchymal stem cells (hucMSCs) in the treatment of dextran sulfate sodium (DSS)-induced inflammatory bowel disease (IBD).

RESULTS:

ICG-hucMSCs homed to colon tissues of IBD mice 12 h after injection. The injection of hucMSCs significantly relieved the IBD symptoms and inflammatory cell infiltration. The expression of IL-10 gene increased while those of 15-LOX-1, TNF-α, IL-6, IL-1β, and IP-10 genes decreased in colon tissues and spleens of hucMSCs-treated mice. The activation of STAT3 was inhibited in colon tissues and spleens of IBD mice that were treated with hucMSCs. In addition, the percentage of macrophages decreased in colon tissues and spleens of hucMSCs-treated IBD mice. Moreover, we provided evidence that in vitro co-culture with hucMSCs inhibited the expression of 15-LOX-1, IL-6 and p-STAT3 in mouse enterocoelia macrophages.

CONCLUSIONS:

HucMSCs alleviate DSS-induced IBD through the modulation of 15-LOX-1 in macrophages.