Biochem Cell Biol. 2017 Mar 17. doi: 10.1139/bcb-2016-0224. [Epub ahead of print]

Can mesenchymal stem cells pretreated with platelet rich plasma modulate tissue remodeling in a rat burn?

Hosni H¹, Rashed LA², Mahfouz S³, Elsayed Hussein R⁴, Alkaffas M⁵, Mostafa S⁶, Abusree A⁷.

Abstract

AIM:

To study the effect of PRP on BM-MSCs proliferation and to investigate their role on healing of experimental burn injury and possible mechanism of action.

METHOD:

Our work divided into in vitro and in vivo studies. In vitro study included,: MSCs and MSCs treated with PRP. TGF-β level and cell proliferation were assesed. In vivo study, 72 rats divided equally into 6 groups: control, burn, burn with MSCs, burn with PRP, burn with both MSCs and PRP and burn with MSCs pretreated with PRP. On the 7th and 20th day of injury, serum TGF-β, TNFα and IL-10 by ELISA, in skin tissue: histopathology and gene expression of MMP-1, TIMP-2, Ang-1, Ang-2 and Vimentin by RT- PCR were performed in all groups.

RESULTS:

In vitro; MSCs proliferation and TGF-β increased in the PRP treated group compared to the control. In vivo; Ang-1, Ang-2 and vimentin were upregulated, while MMP-1 and TIMP-2 were downregulated. TGFβ and IL-10 were increased, whereas TNF-α was decreased in all treated groups with more significance in MSCs and PRP on day 20. Histopathology of burn skin was improved in all treated groups particularly MSCs pretreated with PRP 20 days post burn.