Bull Exp Biol Med. 2017 Feb;162(4):528-533. doi: 10.1007/s10517-017-3654-9. Epub 2017 Feb 27.

Human Umbilical Cord Blood Serum: Effective Substitute of Fetal Bovine Serum for Culturing of Human Multipotent Mesenchymal Stromal Cells.

Romanov YA^1,2, Balashova EE^3,4, Volgina NE⁵, Kabaeva NV³, Dugina TN⁴, Sukhikh GT⁵.

Abstract

Optimal conditions for culturing of multipotent mesenchymal stromal cells in the presence of pooled umbilical cord blood serum were determined. It was found that umbilical cord blood serum in a concentration range of 1-10% effectively supported high viability and proliferative activity of cells with unaltered phenotype and preserved multilineage differentiation capacity. The proposed approach allows avoiding the use of xenogenic animal sera for culturing of multipotent mesenchymal stromal cells and creates prerequisites for designing and manufacturing safe cellular and/or acellular products for medical purposes.