Stem Cell Res. 2017 Jan;18:60-63. doi: 10.1016/j.scr.2016.12.015. Epub 2016 Dec 9.

Generation and characterization of human iPSC line from CD34+ cells isolated from umbilical cord blood belonging to Indian origin.

Fernandes S¹, Talwadekar M¹, Agarwal R², Nair V³, Kale V¹, Limaye L⁴.

Abstract

We describe here the reprogramming of CD34⁺ cells isolated from umbilical cord blood obtained after full term delivery of a healthy female child of Indian origin. The cells were nucleofected by episomal vectors expressing Oct4, Sox2, L-Myc, Klf4, Lin28 and p53DD (negative mutation in p53). Colonies were identified by alkaline phosphatase staining and characterized for expression of pluripotency markers at protein level by immunofluorescence, flow cytometry and at transcript level by PCR. Genomic stability of the cell line was checked by G-banded karyotype. The ability to differentiate to endoderm, mesoderm and ectoderm in vitro was confirmed by immunofluorescence staining.