Stem Cell Reports. 2017 Oct 4. pii: S2213-6711(17)30382-X. doi: 10.1016/j.stemcr.2017.08.025. [Epub ahead of print]

Efficient Large-Scale 2D Culture System for Human Induced Pluripotent Stem Cells and Differentiated Cardiomyocytes.

Tohyama S¹, Fujita J², Fujita C³, Yamaguchi M³, Kanaami S³, Ohno R³, Sakamoto K⁴, Kodama M⁵, Kurokawa J⁶, Kanazawa H³, Seki T³, Kishino Y³, Okada M³, Nakajima K³, Tanosaki S³, Someya S³, Hirano A⁷, Kawaguchi S⁷, Kobayashi E⁸, Fukuda K³.

Abstract

Cardiac regenerative therapies utilizing human induced pluripotent stem cells (hiPSCs) are hampered by ineffective large-scale culture. hiPSCs were cultured in multilayer culture plates (CPs) with active gas ventilation (AGV), resulting in stable proliferation and pluripotency. Seeding of 1 × 10⁶ hiPSCs per layer yielded 7.2 × 10⁸ hiPSCs in 4-layer CPs and 1.7 × 10⁹ hiPSCs in 10-layer CPs with pluripotency. hiPSCs were sequentially differentiated into cardiomyocytes (CMs) in a two-dimensional (2D) differentiation protocol. The efficiency of cardiac differentiation using 10-layer CPs with AGV was 66%-87%. Approximately 6.2-7.0 × 10⁸ cells (4-layer) and 1.5-2.8 × 10⁹ cells (10-layer) were obtained with AGV. After metabolic purification with glucose- and glutamine-depleted and lactate-supplemented media, a massive amount of purified CMs was prepared. Here, we present a scalable 2D culture system using multilayer CPs with AGV for hiPSC-derived CMs, which will facilitate clinical applications for severe heart failure in the near future.