Bull Exp Biol Med. 2017 Nov 25. doi: 10.1007/s10517-017-3971-z. [Epub ahead of print]

Formation of Tissue-Engineered Construct of Human Cartilage Tissue in a Flow-Through Bioreactor.

Sevastianov VI¹, Basok YB², Grigor'ev AM², Kirsanova LA², Vasilets VN².

Abstract

We performed culturing of a cell-engineered construct of human cartilage tissue consisting of biopolymer microstructured collagen-containing hydrogel, human adipose tissue mesenchymal stromal cells, and induction chondrogenic culture medium in a specially designed flow-through bioreactor. On day 16 of the experiment, human adipose tissue mesenchymal stromal cells acquired flattened shape typical for chondroblasts, demonstrated high proliferative activity, and formed extracellular matrix. The observed histological changes in the cultured system attested to the beginning of the formation of a tissue-engineered construct of human cartilage tissue.