Proteomics Clin Appl. 2017 Nov 16. doi: 10.1002/prca.201700082. [Epub ahead of print]

Metabolic Signature of Microvescicles from Umbilical Cord Mesenchimal Stem Cells of Preterm and Term Infants.

Bruschi M¹, Santucci L¹, Ravera S², Bartolucci M³, Petretto A³, Calzia D², Ghiggeri G¹, Ramenghi LA⁴, Candiano G¹, Panfoli I².

Abstract

PURPOSE:

Microvesicles, 200-1000 nm bodies budding from the cell plasma membrane, are a promising source of biomarkers. This study aimed at comparing the proteome of Microvesicles collected by ultracentrifugation from cultured Mesenchymal Stem Cells from Human Umbilical Cord of Preterm newborns(<34-weeks gestational age) in comparison to infants at Term(≥37 weeks). This discovery study was designed to establish the signature of prematurity.

EXPERIMENTAL DESIGN:

Orbitrap mass spectrometry, statistical, bioinformatics and biochemical analyses were employed.

RESULTS:

3253 proteins were identified,78.3% matching among Preterm and Term. Principal component dimensional analyses showed that the two proteomes cluster separately. Cytoscape analysis showed thatthe top gene signatures cluster around inflammation and oxidative metabolism. Both Preterm and Term Microvesicles consumed oxygen, and express ATP synthase and Cytochrome oxidase, but only Preterm MVs synthesized ATP. The gene signature of Preterm condition mainly cluster around inflammation and metabolism.

CONCLUSIONS AND CLINICAL RELEVANCE:

Microvesicles from mesenchymal stem cells conduct aerobic metabolism similarly to exosomes from the same cells, with interesting differences related to their biogenesis and function. The clinical relevance of the study lays in the perspective to utilize Microvesicles as promising sensor of the inflammatory and metabolic state of the preterm newborn, to help in preventing the complications of prematurity. This article is protected by copyright. All rights reserved.

This article is protected by copyright. All rights reserved.