Cytotherapy. 2018 Jan 31. pii: S1465-3249(18)30007-0. doi: 10.1016/j.jcyt.2018.01.002. [Epub ahead of print]

Derivation of male germ cells from induced pluripotent stem cells by inducers: A review.

Amini Mahabadi J¹, Sabzalipoor H², Kehtari M³, Enderami E⁴, Soleimani M⁵, Nikzad H⁶.

Abstract

Induced pluripotent stem cells (iPSCs) refer to stem cells that are artificially produced using a new technology known as cellular reprogramming, which can use gene transduction in somatic cells. There are numerous potential applications for iPSCs in the field of stem cell biology becauase they are able to give rise to several different cell features of lineages such as three-germ layers. Primordial germ cells, generated via in vitro differentiation of iPSCs, have been demonstrated to produce functional gametes. Therefore, in this review we discussed past and recent advances in the in vitro differentiation of germ cells using pluripotent stem cells with an emphasis on iPSCs. Although this domain of research is still in its infancy, exploring development mechanisms of germ cells is promising, especially in humans, to promote future reproductive and developmental engineering technologies. While few studies have evaluated the ability and efficiency of iPSCs to differentiate toward male germ cells in vitro by different inducers, the given effect was investigated in this review.