Dent Mater J. 2018 Mar 17. doi: 10.4012/dmj.2016-329. [Epub ahead of print]

Influence of diameter of fiber membrane scaffolds on the biocompatibility of hPDL mesenchymal stromal cells.

Suarez-Franco JL¹, Vázquez-Vázquez FC¹, Pozos-Guillen A², Montesinos JJ³, Alvarez-Fregoso O⁴, Alvarez-Perez MA¹.

Abstract

This study evaluated the influence in the biocompatibility of human periodontal ligament (hPDL) mesenchymal stromal cell onto poly lactic-acid (PLA) films and PLA fiber membrane. Fiber scaffold was prepared via air jet spinning (AJS) from PLA solutions (6, 7, and 10%) and analyzed using SEM, AFM and FTIR. Biocompatibility was evaluated by adhesion, proliferation and cell-material interaction. PLA film exhibited a smooth and homogenously surface topography in comparison with random orientation of PLA fiber with roughness structure where diameter size depends on PLA solution. Moreover, cell adhesion; proliferation and cell-material interaction has the best respond on random orientation nanofiber of 10, followed by 7, and 6% of PLA in comparison with PLA films. It could be concluded that AJS is an attractive alternative technique for manufacture fiber scaffolds with a tunable random orientation geometry of fibers that allow to produce interconnected porous formed by nanometric fiber diameter structures that could be a potential scaffold for periodontal tissue engineering applications.