ACS Appl Mater Interfaces. 2018 Mar 22. doi: 10.1021/acsami.7b19718. [Epub ahead of print]

Cell-sheet-derived ECM coatings and their effects on BMSCs responses.

Wang X, Chen Z, Zhou B, Duan X, Weng WJ, Cheng K, Wang H, Lin J.

Abstract

Extracellular matrix (ECM) provides a dynamic and complex environment to determine the fate of stem cells. In this work, in vitro cultured cell sheets were treated with paraformaldehyde or ethanol and eventually become ECM. Such ECM was then immobilized on titanium substrates via polydopamine chemistry. Their effects on bone marrow mesenchymal stromal cells (BMSCs) behaviors were investigated. It was found that paraformaldehyde treated ECM coatings (PT-ECM) showed well-maintained microstructure, whereas that of ethanol treated (ET-ECM) were completely changed. As a result, different amide structures and distributions of ECM components, such as laminin and collagen I, were exhibited. Alkaline phosphatase activity, osteocalcin secretion, related gene expression and mineral deposition were evaluated for BMSCs cultured on both ECM coatings. PT-ECM was demonstrated to promote osteogenic differentiation much more efficiently than ET-ECM. That is ascribed to the preservation of native ECM milieu of PT-ECM. Such ECM acquirement and immobilization method could establish surfaces being able to direct stem cell responses on various materials. That shows promising potential in bone tissue engineering and other related biomedical applications.