Stem Cell Reports. 2018 Mar 21. pii: S2213-6711(18)30103-6. doi: 10.1016/j.stemcr.2018.02.012. [Epub ahead of print]

Human iPSC-Derived Endothelial Cells and Microengineered Organ-Chip Enhance Neuronal Development.

Sances S¹, Ho R², Vatine G², West D², Laperle A², Meyer A², Godoy M², Kay PS², Mandefro B³, Hatata S², Hinojosa C⁴, Wen N⁴, Sareen D³, Hamilton GA⁴, Svendsen CN⁵.

Abstract

Human stem cell-derived models of development and neurodegenerative diseases are challenged by cellular immaturity in vitro. Microengineered organ-on-chip (or Organ-Chip) systems are designed to emulate microvolume cytoarchitecture and enable co-culture of distinct cell types. Brain microvascular endothelial cells (BMECs) share common signaling pathways with neurons early in development, but their contribution to human neuronal maturation is largely unknown. To study this interaction and influence of microculture, we derived both spinal motor neurons and BMECs from human induced pluripotent stem cells and observed increased calcium transient function and Chip-specific gene expression in Organ-Chips compared with 96-well plates. Seeding BMECs in the Organ-Chip led to vascular-neural interaction and specific gene activation that further enhanced neuronal function and in vivo-like signatures. The results show that the vascular system has specific maturation effects on spinal cord neural tissue, and the use of Organ-Chips can move stem cell models closer to an in vivo condition.