Bull Exp Biol Med. 2018 Jul 13. doi: 10.1007/s10517-018-4174-y. [Epub ahead of print]

Cytogenetic Analysis of the Results of Genome Editing on the Cell Model of Parkinson's Disease.

Vetchinova AS¹, Simonova VV¹, Novosadova EV², Manuilova ES², Nenasheva VV², Tarantul VZ², Grivennikov IA², Khaspekov LG¹, Illarioshkin SN³.

Abstract

We performed a cytogenetic analysis of the results of CRISPR/Cas9-correction of G2019S mutation in LRRK2 gene associated with Parkinson's disease. Genome editing was performed on induced pluripotent stem cells derived from fibroblasts of a patient carrying this mutation. A mosaic variant of tetraploidy 92 XXYY/46,XY (24-43% cells from various clones) was found in neuronal precursors differentiated from the induced pluripotent stem cells after gene editing procedure. Solitary cases of translocations and chromosome breaks were observed. These data confirm the importance of the development of new approaches ensuring genome stability in CRISPR/Cas9-edited cultures.