Trends Mol Med. 2018 Jun 23. pii: S1471-4914(18)30118-7. doi: 10.1016/j.molmed.2018.06.001. [Epub ahead of print]

iPSC-Derived Enterocyte-like Cells for Drug Absorption and Metabolism Studies.

Macedo MH¹, Araújo F², Martínez E³, Barrias C¹, Sarmento B⁴.

Abstract

Intestinal cell models have been widely studied and used to evaluate absorption and metabolism of drugs in the small intestine, constituting valuable tools as a first approach to evaluate the behavior of new drugs. However, such cell models might not be able to fully predict the absorption mechanisms and metabolic pathways of the tested compounds. In recent years, induced pluripotent stem cells (iPSCs) differentiated into enterocyte-like cells have been proposed as more biorelevant intestinal models. In this review, we describe mechanisms underlying the differentiation of iPSCs into enterocyte-like cells, appraise the usefulness of these cells in tridimensional intestinal models, and discuss their suitability to be used in the future for drug screening.