In Vitro Cell Dev Biol Anim. 2018 Aug 15. doi: 10.1007/s11626-018-0278-y. [Epub ahead of print]

A method for expansion of T cells from cynomolgus monkey (Macaca fascicularis).

Gao C¹, Song Q², Zhang M³, Li J⁴, Yi M², Dong J⁵.

Abstract

T cells have been successfully applied to cancer immunotherapy. However, a challenge is an expansion of T cells from cynomolgus monkey, which is a valuable non-human primate model for T cell therapy transferring to the clinic. Here, we compared several strategies to expand cynomolgus monkey T cell and developed an appropriate method. Our study demonstrated that 100 ng/ml CD3 mAb + 1% PHA+ 1000 U/ml IL2 therapy significantly expanded peripheral blood CD3+ T cells without compromising T cell phenotype in vitro. The results of this study could be used for T cell remodeling, which has significant therapeutic potential in Chimeric Antigen Receptor-T (CAR-T) cell immunotherapy.