Dev Dyn. 2018 Aug 14. doi: 10.1002/dvdy.24663. [Epub ahead of print]

Effective differentiation of induced pluripotent stem cells into dental cells.

Kim EJ¹, Yoon KS¹, Arakaki M², Otsu K³, Fukumoto S², Harada H³, Green DW¹, Lee JM¹, Jung HS^1,4.

Abstract

BACKGROUND:

A biotooth is defined as a complete living tooth, made in laboratory cultures from a spontaneous interplay between epithelial and mesenchymal cell-based frontal systems. A good solution to these problems is to use iPSCs. However, no one has yet formulated culture conditions that effectively differentiate iPSCs into dental epithelial and dental mesenchymal cells phenotypes analogous to those present in tooth development.

RESULTS:

Here, we tried to induce differentiation methods for dental epithelial cells and dental mesenchymal cells from iPSCs. For the dental epithelial cell differentiation, the conditional media of SF2 dental epithelial cells was adjusted to embryoid body (EB). Moreover, we now report on a new cultivation protocol, supported by transwell membrane cell culture that make it possible to differentiate iPSCs into dental epithelial and mesenchymal cells with abilities to initiate the first stages in de novo tooth formation.

CONCLUSION:

Implementation of technical modifications to the protocol that maximize the number and rate of iPSC differentiation, into mesenchymal and epithelial cell layers, will be the next step towards growing an anatomically accurate biomimetic tooth organ. This article is protected by copyright. All rights reserved.

© 2018 Wiley Periodicals, Inc.