Cell Biol Int. 2019 Feb 21. doi: 10.1002/cbin.11119. [Epub ahead of print]

Adipose tissue -derived mesenchymal stem cells and keratinocytes co-culture on Gelatin/Chitosan/β- Glycerol Phosphate Nanoscaffold in skin regeneration.

Lotfi M^1,2, Naderi-Meshkin H³, Mahdipour E⁴, Mafinezhad A⁵, Bagherzadeh R⁶, Sadeghnia HR⁷, Esmaily H⁸, Maleki M⁹, Hasssanzadeh H³, Ghayaour-Mobarhan M¹⁰, Bidkhori HR³, Bahrami AR^3,11.

Abstract

Using cell-based engineered skin is an emerging strategy for treating difficult-to-heal wounds To date, much endeavor has been devoted to the -fabrication of appropriate scaffolds with suitable biomechanical properties to support cell viability and growth in the microenvironment of a wound. The aim of this research was to assess the impact of adipose tissue-derived mesenchymal stem cells (AD-MSCs) and keratinocytes on Gelatin/Chitosan/β-Glycerol Phosphate (GCGP) nanoscaffold in full-thickness excisional skin wound healing of rats. . For this purpose, AD-MSCs and keratinocytes were isolated from rats and GCGP nanoscaffolds were electros-pun. Through an in vivo study, the percentage of wound closure was assessed on days 7, 14, and 21 after wound induction. Samples were taken from the wound sites in order to evaluate the density of collagen fibers and vessels at 7 and 14 days. Moreover, sampling was done on days 7 and 14 from wound sites to assess the density of collagen fibers and vessels. The wound closure rate was significantly increased in the keratinocytes-ADMSCs-scaffold (KMS) group compared with other groups. The expressions of VEGF, collagen type 1, and CD34 were also significantly higher in the KMS group compared to the other groups. These results suggest that the combination of AD-MSCs and keratinocytes seeded onto GCGP nano scaffold provides a promising treatment for wound healing.