Stem Cells Transl Med. 2019 Apr 23. doi: 10.1002/sctm.18-0182. [Epub ahead of print]

Priming Dental Pulp Stem Cells from Human Exfoliated Deciduous Teeth with Fibroblast Growth Factor-2 Enhances Mineralization Within Tissue-Engineered Constructs Implanted in Craniofacial Bone Defects.

Novais A^1,2, Lesieur J¹, Sadoine J¹, Slimani L¹, Baroukh B¹, Saubaméa B³, Schmitt A⁴, Vital S^1,2, Poliard A¹, Hélary C⁵, Rochefort GY¹, Chaussain C^1,2, Gorin C^1,2.

Abstract

The craniofacial area is prone to trauma or pathologies often resulting in large bone damages. One potential treatment option is the grafting of a tissue-engineered construct seeded with adult mesenchymal stem cells (MSC). The dental pulp appears as a relevant source of MSC as dental pulp stem cells display strong osteogenic properties, and are efficient at bone formation and repair. Fibroblast growth factor-2 (FGF-2) and/or hypoxia primings were shown to boost the angiogenesis potential of dental pulp stem cells from human exfoliated deciduous teeth (SHED). Based on these findings, we hypothesized here that these primings would also improve bone formation in the context of craniofacial bone repair. We found that both hypoxic and FGF-2 primings enhanced SHED proliferation and osteogenic differentiation into plastically compressed collagen hydrogels, with a much stronger effect observed with the FGF-2 priming. After implantation in immunodeficient mice, the tissue-engineered constructs seeded with FGF-2 primed SHED mediated faster intramembranous bone formation into critical size calvarial defects than the other groups (no priming and hypoxia priming). The results of this study highlight the interest of FGF-2 priming in tissue engineering for craniofacial bone repair. Stem Cells Translational Medicine 2019;00:1-14.