Methods Mol Biol. 2019;2017:59-70. doi: 10.1007/978-1-4939-9574-5_5.

Transmigration Assays for the Determination of Molecular Interactions Between Hematopoietic Stem Cells and Niche Cells.

Henschler R¹, Richter R^2,3.

Abstract

The transmigration capacity of hematopoietic stem and progenitor cells (HSPC) is characteristically associated with their ability to home to sites of hematopoiesis in the transplanted host, to proliferate, to differentiate, and to successfully repopulate the hematopoietic system of a transplanted host. Stimulating agents shown to induce the transmigration of HSPC were often later identified to play significant roles in mobilization of HSPC or their interaction with niche cells in the hematopoietic microenvironment. Transwell migration assays through microporous membranes have been developed in various forms to determine the migration capacity of HSPC or mesenchymal stromal cells (MSCs) toward chemoattractants. We describe here a method of a multi-well reusable transmigration assay using a small volume and low numbers of HSPC, allowing the simple and reproducible determination of HSPC transmigration capacity which enable researchers to obtain rapid answers at limited costs with high reliability.