Proteomics. 2019 Jul 18:e1800166. doi: 10.1002/pmic.201800166. [Epub ahead of print]

Placenta stem/stromal cell-derived extracellular vesicles for potential use in lung repair.

Kim SY^1,2, Joglekar MV³, Hardikar AA³, Phan TH^1,2, Khanal D^1,2, Tharkar P^1,2, Limantoro C^1,2, Johnson J⁴, Kalionis B⁴, Chrzanowski W^1,2.

Abstract

Many acute and chronic lung injuries are incurable and rank as the fourth leading cause of death globally. While stem cell treatment for lung injuries is a promising approach, there is growing evidence that the therapeutic efficacy of stem cells originates from secreted extracellular vesicles (EVs). Consequently, EVs are emerging as next-generation therapeutics. While EVs are extensively researched for diagnostic applications, their therapeutic potential to promote tissue repair is not fully elucidated. By housing and delivering tissue-repairing cargo, EVs refine the cellular microenvironment, modulate inflammation, and ultimately repair injury. Here, we present the potential use of EVs derived from two placental mesenchymal stem/stromal cell (MSC) lines; a chorionic MSC line (CMSC29) and a decidual MSC cell line (DMSC23) for applications in lung diseases. Functional analyses using in vitro models of injury demonstrated that these EVs have a role in ameliorating injuries caused to lung cells. We also showed that EVs promote repair of lung epithelial cells. This study is fundamental to advancing the field of EVs and to unlock the full potential of EVs in regenerative medicine. This article is protected by copyright. All rights reserved.

This article is protected by copyright. All rights reserved.