World J Plast Surg. 2019 Sep;8(3):352-358. doi: 10.29252/wjps.8.3.352.

Extraction and Characterization of Collagen with Cost-Effective Method from Human Placenta for Biomedical Applications.

Karami A^1,2, Tebyanian H², Sayyad Soufdoost R³, Motavallian E⁴, Barkhordari A¹, Nourani MR¹.

Abstract

BACKGROUND:

Collagen is the main product in pharmaceutics and food industry with a high demand. Collagen can be extracted from several tissues such as skin, bone and tendon, etc. Collagen can be used in tissue engineering researches as a substrate of wound healing and nerve regeneration. Extraction methods of collagen are various with different purities. In this research, we aimed to extract collagen from human placenta with a modified method.

METHODS:

This modified approach was used for extracting of collagen from human placenta with acetic acid and NaCl treatment using different concentrations.

RESULTS:

SDS page showed three different bands that reflected two alpha-chains and one beta-chain with molecular weights of 102, 118 and 220 kDa, respectively. There was no significant difference between extracted collagen from human placenta and standard collagen in western blot analysis.

CONCLUSION:

It was concluded that human placenta can be an alternative source of collagen with high purity for biomedical applications such as tissue engineering, stem cell therapy and research.