Int J Biol Macromol. 2020 Jan 14. pii: S0141-8130(19)37419-7. doi: 10.1016/j.ijbiomac.2020.01.112. [Epub ahead of print]

Fabrication and characterization of dextran/nanocrystalline β-tricalcium phosphate nanocomposite hydrogel scaffolds.

Ghaffari R¹, Salimi-Kenari H², Fahimipour F³, Rabiee SM⁴, Adeli H¹, Dashtimoghadam E⁵.

Abstract

Design of bioactive three-dimensional scaffolds to support bone tissue repair and regeneration become a key area of research in tissue engineering. Herein, porous hybrid hydrogels composed of dextran incorporated with nanocrystalline β-tricalcium phosphate (β-TCP) particles were tailor made as scaffolds for bone tissue engineering. β-TCP was successfully introduced within the dextran networks crosslinked through intermolecular ionic interactions and hydrogen bonding confirmed by FTIR spectroscopy. The effect of β-TCP content on equilibrium water uptake and swelling kinetics of composite hydrogels was investigated. It was found that the homogeneous distribution of β-TCP nanoparticles through the hydrogel matrix contributes to higher porosity and swelling capacity. In depth swelling measurements revealed that while in the early stage of swelling, water diffusion follows the Fick's law, for longer time swelling behavior of hydrogels undergo the second order kinetics. XRD measurements represented the formation of apatite layer on the surface of nanocomposite hydrogels after immersion in the SBF solution, which implies their bioactivity. Cell culture assays confirmed biocompatibility of the developed hybrid hydrogels in vitro. The obtained results converge to offer dextran/β-TCP nanocomposite hydrogels as promising scaffolds for bone regeneration applications.