Biosci Rep. 2020 Mar 31. pii: BSR20181829. doi: 10.1042/BSR20181829. [Epub ahead of print]

Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells.

Lee J¹, Byeon JS¹, Gu NY¹, Lee S¹, Lee SA¹, Jeong DU¹, Ouh IO¹, Cho IS¹, Song JY¹, Lee YH¹, Hyun BH¹.

Abstract

Mesenchymal stem cells (MSCs) possess the ability to differentiate into multiple cell lineages, and thus, confer great potential for use in regenerative medicine and biotechnology. In this study, we attempted to isolate and characterize bovine tongue tissue-derived MSCs (boT-MSCs) and investigate the culture conditions required for long-term culturing of boT-MSCs. boT-MSCs were successfully isolated by the collagenase digestion method and their proliferative capacity was maintained for up to 20 or more passages. We observed a significant increase in the proliferation of boT-MSCs during the 20 consecutive passages under low glucose DMEM culture condition among the three culture conditions. These boT-MSCs presented pluripotency markers (Oct3/4 and Sox2) and cell surface markers, which included CD13, CD29, CD44, CD73, CD90, CD105, CD166, and MHC-I but not CD11b, CD14, CD31, CD34, CD45, CD80, CD86, CD106, CD117, and MHC-II at third passage. Moreover, these boT-MSCs could differentiate into mesodermal (adipocyte, osteocyte, and chondrocyte) cell lineages. Thus, this study suggests that the tongue of bovines could be used as a source of bovine MSCs.