J Chin Med Assoc. 2020 Mar 18. doi: 10.1097/JCMA.0000000000000301. [Epub ahead of print]

Enhancing iPSC Toward Differentiation into Functional Cardiomyocytes.

Chien CS^1,2,3, Leu HB^1,4, Wang CY^2,5, Chien Y^1,2,3, Wang CL¹, Tai HY¹, Ko YL^1,2, Tsai FT^1,2, Chou SJ^1,6, Yu WC^2,7, Yang MY^8,9,10,11.

Abstract

BACKGROUND:

Heart diseases, especially myocardial ischemia, remain one of the leading causes of mortality worldwide and usually result in irreparable cardiomyocyte damage and severe heart failure. Recent advances in induced pluripotent stem cell (iPSC) technologies for applied regenerative medicine and stem cell research specifically for iPSC-derived cardiomyocytes have increased the hope for heart repair. However, the driver molecules of myocardial differentiation and the functional reconstruction capacity of iPSC-derived cardiomyocytes are still questionable.

METHODS:

Herein we established a rapid differentiated platform that is involved in cardiomyogenic differentiation and maturation from iPSCs in vitro. Functional analysis is performed in miR-181a-transfected iPSC-derived cardiomyocyte (iPSC-cardio/miR-181a) under a time-lapse microscope. In addition, we calculated the beating area and frequency of iPSC-cardio/miR-181a cells in the presence of HCN4 shRNA or miR-181a SPONGE.

RESULTS:

miR-181a enhanced the beating area and maintained the beating frequency of iPSC-derived cardiomyocytes by enhancing HCN4 expression.

CONCLUSION:

miR-181a would play a key role on maintaining proper beating function in iPSC-derived cardiomyocytes.