Iran Biomed J. 2020 Feb 12. doi: 10.29252/ibj.24.4.236. [Epub ahead of print]

Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber.

Shapourzadeh A¹, Atyabi SM², Irani S³, Bakhshi H⁴.

Abstract

BACKGROUND:

Through combining two synthetic and natural polymers, scaffolds can be developed for tissue engineering and regenerative medicine purposes.

METHODS:

In this work, CMC (20%) was grafted to PCL nanofibers using the cold atmospheric plasma of helium. The PCL scaffolds were exposed to CAP, and functional groups were developed on the PCL surface.

RESULTS:

The results of FTIR confirmed CMC (20%) graft on PCL scaffold. The MTT assay showed a significant enhancement (p < 0.05) in the cell affinity and proliferation of ADSCs to CMC20%-graft-PCL scaffolds. After 14 days, bone differentiation was affirmed through alizarin red and calcium depositions.

CONCLUSION:

Based on the results, the CMC20%-graft-PCL can support the proliferation of ADSCs and induce the differentiation into bone with longer culture time.