Stem Cell Res Ther . 2020 Feb 7;11(1):54. doi: 10.1186/s13287-020-1555-8.

Different Approaches for Transformation of Mesenchymal Stem Cells Into Hepatocyte-Like Cells

Afsoon Afshari  ¹ , Sara Shamdani  ^{2   3   4} , Georges Uzan  ² , Sina Naserian  ^{2   3   4} , Negar Azarpira  ⁵

Abstract

Due to the prominent role of the liver in the body and detoxification, its functionality can be affected in an irreversible manner by diseases. This phenomenon renders the liver to stop working, leading to morbidity and mortality. Therefore, liver transplantation is the only way to tackle this issue.In order to compensate for the lack of adequate healthy liver tissue for transplantation, therapeutic approaches such as hepatocyte transplantation have been proposed as an alternative. Recognizing the fact that mesenchymal stem cells are adult stem cells with the capacity to differentiate into several cell types, different methods have been invented to produce hepatocyte-like cells from mesenchymal stem cells. They can be divided into three main categories, such as addition of cytokines and growth factors, genetic modifications, and adjustment of microenvironment as well as physical parameters.In this review, we attempted to introduce diverse efficient methods for differentiating mesenchymal stem cells and their capability for transformation into hepatocyte-like cells.