In Vivo. 2014 09-10;28(5):733-739.

Bone Substitutes Enhance Osteogenic Differentiation of Mesenchymal Stem Cells in Three-dimensional Scaffolds.

Hanken H¹, Alpers J², Böbel M³, Wöltje M³, Hartjen P², Friedrich RE², Jung O², Rendenbach C², Gröbe A², Heiland M², Al-Dam A², Eichhorn W², Smeets R².

Abstract

Aim: The present study aimed to find bone substitutes to enhance osteogenic differentiation of mesenchymal stem cells in three-dimensional scaffolds in the absence of dexamethasone.

 Materials and Methods: Seven commercial bone substitutes were added to a three-dimensional fibrin-matrix containing rat mesenchymal stem cells in a biocompatible poly-L-lactic-acid mesh. Cell viability, cytotoxicity and alkaline phosphatase activity were followed for three weeks. Expression of bone markers was examined by qualitative evaluation of corresponding transcripts.

 Results: Six out of the seven bone derivatives exhibited an osteogenic-enhancing effect.

 Conclusion: The osteogenic-enhancing effect of the evaluated bone substitutes suggests their potential clinical application for preparation of autologous bone replacement material in three-dimensional carriers.