The Helmholtz Centre for Infection Research (www.helmholtz-hzi.de) seeks a Research Assistant (PhD student / in) for the working group "Cellular Proteomics" (Head: Prof. Dr. Lothar Jansch) in the Department of Structural Biology, for a joint project with the DKFZ.

The aim of the project "The role of ubiquitination in the by the Epstein-Barr virus-induced proliferation of B cells" is to study the role of ubiquitination and Deubiqutinierungsereignissen in EBV-mediated transformation of B-cells and in the case of propagation these cells.
As part of a research proposal to an established proteomics approach (quantitative peptide sequencing; UPLC Orbitrap Velos MS) responses of human cells to infection with the Epstein-Barr virus are examined. Project focus will be the elucidation of the ubiquitin (Ub) network as a post-translational modification and signal mediator. We plan to qualitative and quantitative analyzes of viral and cellular proteome in dormant, CD40-stimulated and EBV-transformed cells with a focus on ubiquitinated proteins, the identification of new and possibly viral deubiquitinierender proteases (DUBs) in proliferating and EBV-transformed cells and their substrate proteins and the function of validated targets to clarify. As well as modern methods of chemical proteomics cell biology, bioinformatics and pathway-modulating process (si / shRNA, inhibitors) are used in this project are used.
All students of the Institute will be funded as part of the Helmholtz Graduate School.

Prerequisites:
• University degree in biology, biotechnology, biochemistry or related fields
• a basic knowledge of working with proteins and mammalian cells
• Strong motivation and team spirit and willingness to work in an international team
• good knowledge of English, written and spoken

With the same professional capacity with severe disabilities preference.
Equality of opportunity is part of personnel policy, we therefore call for qualified women to apply.

Start date: as soon as possible, initially limited to 3 years

Remuneration: TVÖD E13 (65%)

Probation / training period: 6 months

Closing date: 01.11.2012

Application: https://online-application.helmholtz-hzi.de/

Please refer to when entering their data on the reference number 67/2012

Information: For further information please contact Prof. Lothar Jansch, head of the AG Cellular Proteomics 0531/6181-3030 call or e-mail: lothar.jaensch @ helmholtz-hzi.de.