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PhD position ( molecular biology ): Studying novel DNA modification and DNA editing by MS : Mainz, Germany

In the field of “DNA demethylation and development” the research group of Professor Christof Niehrs offers the following PhD project:

Studying novel DNA modification and DNA editing by mass spectrometry

Description:

Modified DNA bases play an important epigenetic role in the regulation of gene expression in development and disease. In mammals there are four different cytidine modifications in genomic DNA known to have an epigenetic function, most prominently 5-hydroxymethylcotosine. There are many more known DNA modifications, whose function remain unknown and await characterization, including of the cellular enzymes that affect these modifications, which will be the aim of the project. Our lab has a broad expertise not only in molecular cell biology and bioinformatics, but in bioanalytical chemistry, where we are using LC/MS/MS detection for modified nucleobases. Beyond mass spectrometry analysis, we will knockout candidate genes in mouse embryonic stem cells (mESCs) by the CRISPR/Cas9 genome editing technique and analyze the physiological relevance of these genes in pluripotency and during mESC in vitro differentiation by e.g. RNA sequencing. Given the biological importance of the new DNA modification, the project promises to be ground breaking, opening unexplored fields in epigenetics. This PhD project will be particularly suited for students with interest in biochemistry and/or bioorganic analytical methods, particularly mass spectrometry.

We offer

• The possibility to work on a cutting-edge project using state-of-the-art technology in a highly motivated research team

• A stimulating, diverse and international research environment

• Advanced training opportunities

• A competitive stipend

Required qualifications

• Master or Diploma

• Motivation to solve complex biological problems

• Excellent communication skills

Starting date: 1 September 2016 or later

Duration of stipend: 3 years, with the possibility of extension

Deadline for applications (exclusively online via web form):  1 May 2016

Don't forget to mention Naturejobs when applying

Job details

Employer:Institut of Molecular Biology (IMB)

Website:https://www.imb-mainz.de/

Location:Institut für Molekulare Biologie gGmbH, Ackermannweg 4, Mainz, Germany

Expires:May 08, 2016

Job type:PhD Studentship

Salary:Unspecified

Qualifications:Postgraduate - Master's degree

Employment type:Temporary

Job hours:Full-time