www.nature.com/naturejobs

PhD position (molecular biology): Primary Cilia and microRNA: Mainz, Germany

In the field of “Primary cilia and microRNA” the research group of Dr Helen May-Simera offers the following PhD project:
 
The role of primary cilia and their regulation by microRNA in the retinal pigment Epithelium
 
The retinal pigment epithelium (RPE) is a monolayer barrier of highly polarized, pigmented epithelia that supports the light-sensitive photoreceptors of the retina. Correct development of the RPE is essential for visual function. We characterised the primary cilium in the developing mouse RPE, and have shown that temporal expression of these organelles is required for RPE differentiation (May-Simera et al., in preparation). Furthermore we were able to identify possible miRNAs that may influence ciliogenesis in the developing RPE.  However it remains unclear how miRNAs regulate the expression of ciliary genes and how this affects RPE development. The aim of this study is to characterize the influence of miRNAs on ciliogenesis of primary cilia and to decipher the role of primary cilia in polarization and maturation of the RPE. We will perform transcriptomic analysis of coding and small non-coding RNAs during development of the RPE in both control and cilia mutant mice. This will be followed by functional studies in primary mouse RPE explants, in which expression of specific miRNAs and their putative targets have been modulated. These in vitro culture assays will facilitate investigation into the role of miRNAs on cell size, polarity and formation of cilia, and can ascertain the relevance to RPE differentiation and function. Understanding the mechanism regulating the differentiation and function of RPE cells in vivo will be important for improving the current effort to employ these cells for cell-replacement therapies.
 
We offer
• The possibility to work on a cutting-edge project using state-of-the-art technology in a highly motivated research team
• A stimulating, diverse and international research environment
• Advanced training opportunities
• A fully funded PhD Position
 
Required qualifications
• Master or Diploma
• Motivation to solve complex biological problems
• Excellent communication skills
 
Starting date: 1 March 2017 or later
Deadline for applications (exclusively online via web form): 10 November 2016

Don't forget to mention Naturejobs when applying

Job details

Employer:Institut of Molecular Biology (IMB)

Website:https://www.imb-mainz.de/

Location:Institut für Molekulare Biologie gGmbH, Ackermannweg 4, Mainz, Germany

Expires:November 17, 2016

Job type:PhD Studentship

Salary:Unspecified

Qualifications:Postgraduate - Master's degree

Employment type:Temporary

Job hours:Full-time